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Review
. 2000 Jun 23;240(1-2):1-14.
doi: 10.1016/s0022-1759(00)00185-x.

Analysis of human self-reactive antibody repertoires by quantitative immunoblotting

D Stahl  1 , S Lacroix-Desmazes, L Mouthon, S V Kaveri, M D Kazatchkine
Affiliations
Review

Analysis of human self-reactive antibody repertoires by quantitative immunoblotting

D Stahl et al. J Immunol Methods. .

Abstract

We review the use of a quantitative immunoblotting technique to characterize human self-reactive antibody repertoires in health and disease. The interactions of plasma IgM and IgG with tissue extracts as sources of self-antigens were analyzed by quantitative immunoblotting. Data were compared by means of multiparametric statistical analysis. The data summarized here demonstrate that natural self-reactive antibody repertoires of healthy individuals are restricted to a limited subset of immunodominant autoantigens that is selected early in development, and remains conserved between individuals through ageing. The selection of human natural self-reactive IgG antibody repertoires requires normal T-/B-cell interactions. The immunoblotting assay has the potential to distinguish between autoimmune diseases with organ-related oligoclonal expansion of self-reactive clones and those characterized by broad alterations of immunoregulation. However, organ-specific autoimmune diseases may be characterized by altered patterns of antibody repertoires unrelated to the target organ. The assay also revealed an unexpected defect in the regulatory function of self-reactive IgM on the expression of self-reactive IgG repertoires in several systemic and organ-specific autoimmune diseases. The results are discussed in the light of our current understanding of the processes of selection of self-reactive B-cells and the pathophysiology of autoimmunity.

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