Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza
- PMID: 32353252
- PMCID: PMC7189862
- DOI: 10.1016/j.cell.2020.04.020
Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza
Abstract
The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 virus, has highlighted the need for antiviral approaches that can target emerging viruses with no effective vaccines or pharmaceuticals. Here, we demonstrate a CRISPR-Cas13-based strategy, PAC-MAN (prophylactic antiviral CRISPR in human cells), for viral inhibition that can effectively degrade RNA from SARS-CoV-2 sequences and live influenza A virus (IAV) in human lung epithelial cells. We designed and screened CRISPR RNAs (crRNAs) targeting conserved viral regions and identified functional crRNAs targeting SARS-CoV-2. This approach effectively reduced H1N1 IAV load in respiratory epithelial cells. Our bioinformatic analysis showed that a group of only six crRNAs can target more than 90% of all coronaviruses. With the development of a safe and effective system for respiratory tract delivery, PAC-MAN has the potential to become an important pan-coronavirus inhibition strategy.
Keywords: 2019-nCoV; COVID-19; CRISPR; Cas13; IAV; RdRP; SARS-CoV-2; antiviral; influenza; nucleocapsid.
Copyright © 2020 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of Interests The authors have filed provisional patents via Stanford University related to this work.
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Comment in
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CRISPR-Cas13 as an Antiviral Strategy for Coronavirus Disease 2019.CRISPR J. 2020 Jun;3(3):140-142. doi: 10.1089/crispr.2020.29094.yzh. CRISPR J. 2020. PMID: 33560913 No abstract available.
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